2017年7月5日水曜日

Affirmer

Affimer proteins are versatile and renewable affinity reagents.  eLife 6: e24903. 
Molecular recognition reagents are key tools for understanding biological processes and are used universally by scientists to study protein expression, localisation and interactions.  Antibodies remain the most widely used of such reagents and many show excellent performance, although some are poorly characterised or have stability or batch variability issues, supporting the use of alternative binding proteins as complementary reagents for many applications.  Here we report on the use of Affimer proteins as research reagents.  We selected 12 diverse molecular targets for Affimer selection to exemplify their use in common molecular and cellular applications including the (a) selection against various target molecules; (b) modulation of protein function in vitro and in vivo; (c) labelling of tumour antigens in mouse models; and (d) use in affinity fluorescence and super-resolution microscopy.  This work shows that Affimer proteins, as is the case for other alternative binding scaffolds, represent complementary affinity reagents to antibodies for various molecular and cell biology applications. 

学生の頃に研究を始めてから今まで常にいろんな抗体を使って免疫染色をしてきたし、つい最近も数ヶ月かけてようやく出来上がった新しい抗体の使用条件を探っているところなんだけども、実験で使う抗体に代わるものっていつになったら出て来るんかなってずーっと思っていた。このアファーマーっていうのがそれになりうるだろうか。UKのAvactaっていう会社が開発しているようだ。 

"Future challenges are to test the Affimer in even more applications and to encourage its wider use by researchers, alongside other alternative binding proteins, as replacements for some antibodies.  This could ultimately lead to the development of faster and more efficient diagnostic, imaging and therapeutic tests."

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